University Journal of Pre and Paraclinical Sciences
A CORRELATION BETWEEN HBA1C AND SUPEROXIDE DISMUTASE LEVELS IN TYPE II DIABETES MELLITUS.
Abstract
Background. Oxidative stress plays a significant role in the pathogenesis of many diseases, one among which is Diabetes Mellitus. Increased free-radical generation, non-enzymatic protein glycosylation, auto-oxidation of glucose, alteration in antioxidant enzymes, are some of the proposed mechanisms of oxidative stress in diabetes which is implicated in the pathogenesis of diabetic complications. All the above mechanisms are attributed to a single factor i.e. hyperglycemia. Poor glycemic control leads to increased generation of oxygen free radicals and decreased activity of antioxidant enzymes which ultimately causes cell damage. Considering the occurrence of oxidative stress in diabetes, the present investigation was done to find out the correlation between glycemic control using HbA1c levels as an indicator and antioxidant status using superoxide dismutase (SOD) levels as an index of oxidative stress. Materials and methods. 30 patients with type II diabetes mellitus in the age range of 40-60 years and 30 age and sex matched normal healthy subjects were enrolled for the study. After obtaining informed consent, fasting and postprandial plasma glucose, HbA1c and SOD levels were analysed in the blood samples of all the participants. Results. Diabetic patients had a significant higher HbA1c (p 0.00) levels and lower SOD (p 0.00) levels when compared to control subjects. Significant positive correlation (r 0.60, p 0.00) and negative correlation (r - 0.57, p 0.001) was obtained between duration of diabetes and HbA1c, SOD levels respectively in diabetics. Also a significant negative correlation (r - 0.75, p 0.00) was observed between HbA1c and SOD levels in diabetic patients. In diabetics with poor glycemic control, SOD levels were significantly (p 0.002) lower when compared to patients with good glycemic control. Conclusion. Decrease in SOD levels in diabetic patients suggests the presence of oxidative stress especially in patients with poor glycemic control. Glycemic control has a significant influence on antioxidant levels. Hence, good glycemic control prevents the development of oxidative stress which alleviates the long term complications of diabetes mellitus.
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Brownlee M. Biochemistry and molecular cell biology of diabetic complications. Nature. 2001; 414:813 – 820.
Fumiaki Kimuri et al., Serum extracellular SOD in patients with type 2 Diabetes Mellitus; Relationship to the development of micro- and macrovascular complications. Diabetes care. 2003; 26: 1246-50.
Halliwell, B, Gutteridge JMC. Free Radicals in Biology and Medicine. 2nd ed. Clarendon Press, Oxford. 1989.
Hisalkar PJ, Patne AB, Fawade MM. Assessment of plasma antioxidant levels in type 2 diabetes patients. International Journal of Biological & Medical Research. 2012; 3:1796-1800.
John W Baynee. Perspectives in Diabetes. Role of oxidative stress in development of complications in Diabetes. Diabetes. 1991; 40:405-412.
Kangralkar VA, Shivraj D. Patil RM, Bandivadekar. Oxidative stress and diabetes: a review. International Journal of Pharmaceutical Applications. 2010; 1:38-45.
Marica Jandric-Balen, Veljko Bozikov, Jadranka Bozikov, Zeljko Metelko, Ivan Jandric, Zeljko Romic. Impact of glycemic control on antioxidant enzyme activity in patients with type 2 Diabetes Mellitus. Diabetologia croatica. 2004; 33-34.
Mclennan SV, Heffernen S, Wright L. Changes in hepatic glutathione metabolism in diabetes. Diabetes. 1991; 40:344-348.
Mohammed Najikassim. Oxidative stress and metabolic control in type 2 Diabetic patients. 2011.
Moussa SA. Oxidative stress in Diabetes Mellitus. Romanian J. Biophys. 2008; 18:225-236.
Mullarkey CJ, Edelstein D, Brownlee L. Free radical generation by early glycation products: a mechanism for accelerated atherogenesis in diabetes. Biochem. Biophys. Res. Comm. 1990; 173:932-939.
Packer L. Oxidative stress and antioxidants: the antioxidant network, alpha-lipoic acid and diabetes. In: Packer et al., eds. Antioxidants in diabetes management. Newyork: Marcel Dekker, Inc., 2000; 1-15.
Pasupathi P, Manivannan P, Uma M,. Deepa M. Glycated haemoglobin (HbA1c) as a stable indicator of type 2 diabetes. International Journal of Pharmaceutical and Biomedical Research. 2010; 1: 53-56.
Rosanna Mancinelli, Eleonora Barlocci, Sonia Palminiello, Luciano Saso. Oxidative stress and brain diseases: Biomarkers and analytical methodologies. Indian Journal of Biotechnology. 2011; 10:395-403.
Rosen P, Nawroth PP, King G, Moller W, Tritschler HJ, Packer L. The role of oxidative stress in the onset and progression of diabetes and its complications, a summary of a Congress Series sponsored by UNESCO-MCBN, the American Diabetes Association , and the German Diabetes
Society. Diabetes Metab Res Rev. 2001; 17:189-212. 16. Sarita N Shinde, Vithal N Dhadke, Adinath N Suryakar. Evaluation of oxidative stress in Type 2 Diabetes Mellitus and Follow-up along with Vitamin E supplementation. Ind J Clin Biochem. 2011; 26:74-77.
Strain JJ. Disturbances of micronutrient and antioxidant status in diabetes. Proceedings of the Nutrition Society. 1991; 50:591-604.
World Health Organisation. Definition and diagnosis of diabetes mellitus and intermediate hyperglycemia. Report of a WHO/IDF consultation. 2006.
World Health Organisation. Use of Glycated Haemoglobin (HbA1c) in the Diagnosis of Diabetes Mellitus. Abbreviated Report of a WHO Consultation. 2011.
Young IS, Torney JJ, Trimble ER. The effect of ascorbate supplementation on oxidative stress in the streptozocin diabetic rat. Free Radical Biology and Medicine. 1991; 8: 752-758.
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