Evaluation of anxiolytic activity of ethanolic extract of ocimum sanctum leaves in albino mice

Yesodha S .

Abstract


OBJECTIVES To evaluate the anxiolytic property of ethanolic extract of Ocimum sanctum leaves in albino mice. MATERIALS AND METHODS Thirty six inbred male albino mice weighing 20-25 grams were selected from central animal house. For elevated plus maze t est 18 animals were divided into three groups and for open field test 18 animals were divided in to three groups. Each group consist of six animals. The control group received vehicle propylene glycol (2mlkg) orally, standard group received Diazepam (1mgkg, ip) 15 minutes before experimentation and test group received extract of Ocimum sanctum leaves (200mgkg) orally once daily for seven days. Anxiolytic activity assessed by using elevated plus maze and open field test. The results were tabulated and analysed with suitable statistical method. RESULTS Ocimum sanctum leaves showed statistically significant anxiolytic activity at the dose of 200mgkg after seven days of daily administration (P 0.0001). The results were comparable to that produced by standard drug diazepam. CONCLUSION Ocimum sanctum leaves have anxiolytic activity which is comparable to diazepam. Further studies are essential to prove the anti-anxiety activity of Ocimum sanctum in human.

Full Text:

PDF

References


Jerald kay, Allan Tasman. Anxiety Disorders: Generalized Anxiety Disorders. In: Essentials of Psychiatry. John Wiley & Sons Ltd. 2006: p 639- 653.

Julie Loebach Wetherell, Ph.D., and Murray B. Stein, M.D. Anxiety Disorders. In: Benjamin James Sadock, M.d. Virginia Alcott Sadock, M.D. Pedro Ruiz, M.D. Kaplon & Sasock’s Comprehensive Textbook of Psychiatry. 9 th edition. Volume II. USA, Wolters Kluwer/ Lippincott Williams & Wilkins. 2009: p4040- 4047.

Anthony J. Trevor, PhD, & Walter L. Way, MD. Sedative – Hypnotic Drugs. In: Bertram G. Katzung, Susan B. Masters, Anthony J. Trevor. Basic & Clinical Pharmacology. 12 th edition. New Delhi, Tata McGraw hill. 2012: p 373 – 388.

Tripathi K. D. Drugs Used in Mental Illness: Antidepressant and Antianxiety Drugs. In: Essential of Medical Pharmacology. 7th edition. New Delhi: Jaypee Brothers Medical Publishers Ltd. 2013: p 454 – 468.

James M. O’Donnell and Richard C. Shelton. Drug Therapy of Depression and Anxiety Disorders. In: Laurence L.Brunton, Bruce A. Chabner & Bjorn C.Knollmann; Goodman & Gilman’s The Pharmacological basis of therapeutics, 12th edition. New York, McGraw Hill, 2011: p 398- 415.

R.N.Chopra, S.L.Nayar, I.C.Chopra. Glossary of Indian Medicinal Plants. Ocimum sanctum. National Institute of Science Communication and Information Resources, New Delhi.2006: P 178-180.

Kochhar A, Sharma N and Sachdeva R. Effect of Supplementation of Tulsi (Ocimum sanctum) and Neem (Azadirachta indica) Leaf Powder on Diabetic Symptoms, Anthropometric Parameters and Blood Pressure of Non Insulin Dependent Male Diabetics. Ethno-Med 2009, 3(1): p 5-9.

Chiang L C, Ng L T, Cheng P W, Chiang W & Lin C. Antiviral activities of extracts and selected pure constituents of Ocimum basilicum. Clinical and Experimental Pharmacology and Physiology 2005, 32(10): p 811-816.

Karthikeyan, P. Gunasekaran, N. Ramamurthy and S. Govindasamy Anticancer Activity of Ocimum Sanctum. Summary Pharmaceutical Biology 1999, Vol. 37, No. 4, P 285-290.

R.K.Gupta. Medicinal and Aromatic Plants.Ocimum sanctum. 1 st edition, CBS Publishers & Distributors Pvt Ltd. New Delhi.2010: p 277-279.

A.V.S.S.Sammbamurty. Dictionary of Medicinal plants. Ocimum sanctum. 1st edition, CBS Publishers&Distributors, New Delhi. 2006: p 207.

Vinod D. Rangari. Pharmacognosy & Phytochemistry. Part-II, 1st edition, Mr.Nishad Deshmukh, Career Publications. 2004: p250-252.

S.K. Kulkarni. Pharmacology of central nervous system. In: Practical Pharmacology and Clinical Pharmacy. Vallabah publications. New Delhi.2008: p 130-162.

Michel Bourin, Benoit Petit- Demouliere, Brid Nic Dhonnchadha, Matrine Hascoet. Animal models of anxiety in mice. 2007 The Authors journal compilation. Blackwell publishing Ltd. Fundamental & Clinical Pharmacology 21 (2007) p 567-574.

H. Gerhard Vogel. Wolfgang H. Vogel. Drug Discovery and Evaluation- Pharmacological assays. Springer – Verlag Berlin Heidelberg Newyork. 1997: p 213-245.


Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.

An Initiative of The Tamil Nadu Dr M.G.R. Medical University