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Isolated hind limb preparation to study the peripheral vascular resistance



A denervated, isolated rat hind limb model was used to study vascular resistance, one of the major determinants of blood pressure. The aim of this work was to assess the potential usefulness of this model to study vascular properties namely, arteriolar resistance, arterial compliance and impedance. Rat abdominal aorta was cannulated proximal to its bifurcation, pressure transducer was connected and hind limb vasculature was perfused at a constant flow rate with a peristaltic pump. Vascular resistance can be calculated as Mean pressureflow rate as flow is set by the experimenter and pressure is  measured. If flow is kept constant, vascular resistance and  compliance are reflected by changes in mean arterial pressure and pulse pressure respectively. Norepinephrine increased mean pressure, but not pulse pressure therefore it increased resistance, but did not change compliance. High potassium increased mean pressure and pulse pressure Therefore it  increases resistance, as well as decreases compliance. The ease of this technique makes this preparation an ideal tool for research and teaching. In conclusion, this will aid in unravelling mechanisms by which blood vessels helps in regulating blood pressure.


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Peiper U, Griebel L, Wende W. Activation of vascular smooth muscle of rat aorta by noradrenaline and depolarization: Two different mechanisms. Pflüg Arch. 1971 Mar 1;330(1):74–89.

Bolton TB, Lang RJ, Takewaki T. Mechanisms of action of noradrenaline and carbachol on smooth muscle of guinea-pig anterior mesenteric artery. J Physiol. 1984 Jun;351:549–72.

Jerius H BC, Beall A, Karolyi D, Brophy C. Vascular smooth muscle mechanics in isolated perfused segments of carotid arteries. Surg 2000 1272148-54. 2000;

Marra SP, Kennedy FE, Kinkaid JN, Fillinger MF. Elastic and rupture properties of porcine aortic tissue measured using inflation testing. Cardiovasc Eng 20066125-33. 2006;

M. Rosic SP, M. Colic1 , V. Ilic2 , Z. Tepic. Transport and biomechanical studies in the isolated blood vessel segment. J Serbian Soc Comput Mech Vol 5 No 2 2011 Pp 51-58. 2011;

Monson KL, Mathur V, Powell DA. Deformations and end effects in isolated blood vessel testing. J Biomech Eng. 2011 Jan;133(1):011005.

Angus JA, Wright CE. Techniques to study the pharmacodynamics of isolated large and small blood vessels. J Pharmacol Toxicol Methods. 2000 Oct;44(2): 395–407.

Cox RH, Bagshaw RJ. Reflex control of canine hindlimb resistance under different modes of perfusion. Am J Physiol. 1981 Dec;241(6):H789–94.

Hainsworth R, Karim F, McGregor KH, Wood LM. Hind-limb vascular-capacitance responses in anaesthetized dogs. J Physiol. 1983 Apr;337:417–28.

Reitan JA, Kien ND, Martucci RW, Thorup SJ, Dennis PJ. Development of a near anesthetic-free isolated canine hindlimb model. The effects of halothane and atropine sulfate on vascular resistance. J Pharmacol Methods. 1991 Nov;26(3):223–32.

Wu ZY, Rivory LP, Roberts MS. Physiological pharmacokinetics of solutes in the isolated perfused rat hindlimb: characterization of the physiology with changing perfusate flow, protein content, and temperature using statistical moment analysis. J Pharmacokinet Biopharm. 1993 Dec;21(6):653–88.

Bonen A, Clark MG, Henriksen EJ. Experimental approaches in muscle metabolism: hindlimb perfusion and isolated muscle incubations. Am J Physiol. 1994 Jan; 266(1 Pt 1):E1–16.

Casquero-Dorado AC, Sánchez-Navarro A. The isolated hind limb preparation as a drug distribution model. Methods Find Exp Clin Pharmacol. 1996 Sep;18(7):453–8.


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