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BACKGROUND Prevalence of Pseudomonas aeruginosa infection is common among burns patients.  Metallo-beta-lactamases (MBLs) produced by clinical isolates of Pseudomonas aeruginosa has increased considerably in recent years. This may cause phenotypic resistance to virtually all  clinically available bate lactams. The drug resistance due to MBL has a potential for rapid spread to other    microorganisms AIMS AND OBJECTIVES To detect the MBL producing Pseudomonas aeruginosa from burns wound          infections and to study their antibiotic sensitivity                          pattern METHOD Pseudomonas aeruginosa were isolated from infected burns wounds samples. Imipenem resistant isolates were further tested by double disc synergy test (DDST) and Combined Disc Diffusion Test (CDDT).RESULTS Out of total 156 Pseudomonas aeruginosa isolates, 58 (37.1) were imipenem resistant, among which, 42(26.92) isolates were MBL producers by double disc synergy test (DDST) and 46 (29.48) were MBL producers by Combined Disc Diffusion Test (CDDT). MBL producers were resistant to commonly used antibiotics than non MBL producers. All isolates were sensitive to colistin (10micrograms) and polymyxin B (300 units). CONCLUSION MBL production is an important mechanism of carbapenem resistance among Pseudomonas aeruginosa . Double disc  synergy test (DDST) and Imipenem- EDTA Combined Disc  Diffusion Test (CDDT) can be used for detection of MBL Production. So screening method should be done routinely to determine changing trends in the type of organisms and their resistance pattern. Patients infected with MBL producing  organisms should be promptly treated. Antibiotic stewardship programme should be made which is the main stay in the management of burns wound infection and to prevent the spreading of antibiotic resistance.


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