University Journal of Pre and Paraclinical Sciences
A STUDY ON BIOFILM PRODUCTION AMONG ISOLATES OF Proteus species FROM URINE, PUS AND WOUND SAMPLES
Abstract
Background - Proteus spp currently cause 29 percent of nosocomial (hospital-acquired) infections in the United States. These nosocomial pathogens are responsible for 46 percent of urinary tract and 24 percent of surgical site infections. So this study has been chosen to determine biofilm production in Proteus and related species from urine, pus and wound samples as its biofilm determination will lead to a better prevention, and treatment options. Objectives - To isolate clinically significant Proteus and related species from urine, pus and wound samples and to determine Biofilm production by Tissue culture Plate method and also to study the antimicrobial susceptibility of these isolates and compare them with Biofilm production. Materials and methods - 75 clinically significant Proteus isolates from pus, wound and urine samples were identified by standard biochemical tests. All these isolates were subjected to Biofilm determination by Tissue culture plate method. Biofilm was quantitatively measured by Spectrophotometric method. Antimicrobial susceptibility testing was done by disc diffusion method using Kirby Bauer technique on Mueller Hinton agar (HiMedia, Mumbai) ,using appropriate antimicrobial drugs as directed by CLSI guidelines. Results - The clinical isolates included in the present study mostly comprised of Proteus mirabilis (53.3 percent) and Proteus vulgaris (38.6 percent) and (8 percent) of Morganella morgagnii These isolates were most frequently isolated from Pus (57.3 percent) followed by wound and urine samples. 81.3 of isolates were Biofilm producers among which 49.2 percent are strong biofilm and 32 percent are moderate biofilm producers. Multiple drug resistance was significantly higher in Biofilm producers (45.9 percent) as compared to Non Biofilm producers.(P value less than or equal to 0.001). Conclusion - It is evident from the present study that Proteus mirabilis followed by Proteus vulgaris are the most common species isolated from pus specimen. There is an association between biofilm production with drug resistance. Species identification and study of biofilm production and antimicrobial resistance will assist in the management and control of infections.
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