University Journal of Pre and Paraclinical Sciences
BACTERIOLOGICAL PROFILE OF POST OPERATIVE WOUND INFECTIONS AND THEIR ANTIMICROBIAL SUSCEPTIBILITY PATTERN IN A TERTIARY CARE HOSPITAL
Abstract
Introduction - Post operative wound infection
is an important cause of morbidity and
cost burden for the patients. Surveillance
of these data is an important foundation of
effective infection control programs. Aim -
To detect the bacterial profile and evaluate
their antimicrobial sensitivity pattern in
postoperative wound infections and to determine
MRSA among the Staphylococcus
aureus isolates. Materials and Methods - A
total number of 136 isolates were obtained
from 170 wound swabs which were collected
from clinically suspected postoperative
wound infection patients. The
isolates were cultured and identified by
their colony morphology in Blood agar,
MacConkey agar Nutrient agar and speciated
by their standard biochemical reactions.
All the isolates were tested for antimicrobial
sensitivity by Kirby Bauer disc
diffusion method according to the CLSI
guidelines on Mueller Hinton Agar. The
screening and confirmation of MRSA production
was done by OxacillinCefoxitin
disc diffusion method among the
S.aureus isolates. Results - Staphylococcus
aureus (50.7percent) was the predominant
pathogen from post operative
wounds followed by Klebsiella spp
(19.2percent), Escherichia coli
(12.5percent), Proteus spp (8.1percent),
Pseudomonas aeruginosa (6.6percent)
and CONS (2.9percent). Out of 69
Staphylococcus aureus isolates 32
(46.4percent) were detected as MRSA.
The drugs highly effective for GPC were
Vancomycin (100percent) and Gentamicin
(73.9percent). The drugs highly effective
for GNB were Piperacillin Tazobactum
(100percent) and Meropenem
(100percent). Conclusion - This study
concludes that Staphylococcus aureus is
the most common pathogen in post operative
wound infections with increasing
tendency towards MRSA. Since the
S.aureus constitutes the common skin
and nasal flora, stringent monitoring of
aseptic surgical and health care procedures,
pathogen surveillance and effective
anti microbial sensitivity screening are essential
to avoid the poor outcome of surgical
treatment.
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