Detection of Biofilm forming Bacterial isolates from Wound infection and their sensitivity pattern

Abstract

Biofilm forming ability of bacteria resulting in the emergence of drug resistant strains has increased the morbidity and mortality associated with wound infections. The purpose of this study is to isolate bacteria causing wound infection and to detect their biofilm forming ability by Microtitre plate method and to study the sensitivity pattern of the bacterial isolates.A total of 357 bacterial isolates from wound infection were processed, identified and the ability to form biofilm was studied by Microtitre plate method. Antibiotic Susceptibility Testing for Amoxycillin, Erythromycin, Gentamicin, Amikacin, Cephalexin, Cefotaxime, Ceftazidime, Ciprofloxacin, Ofloxacin, Vancomycin, Linezolid, Piperacillin-tazobactum and Imipenem was done by Disc                diffusion method. All Staphylococcus aureus strains were screened for MRSA production. All Klebsiella pneumoniae,  Escherichia coli, Proteus vulgaris strains were studied for ESBL production. Out of 357 isolates 260 (72.8) were biofilm                   producers and 44 (12.32) showed strong biofilm producing  ability. Among the biofilm producers Staphylococcus aureus 83(62.40) was the commonest followed by Klebsiella pneumoniae 56(74.66), Pseudomonas aeruginosa 46(83.63) and Escherichia coli 33(76.74). Among the 357 bacterial isolates Staphylococcus aureus 133(37.25) was the commonest bacteria isolated          followed by Klebsiella pneumoniae 75(21.01). Staphylococcus aureus showed highest sensitivity to Amikacin (82.7) and            ofloxacin (79.69). 74(55.63) were MRSA and showed 100             sensitivity to vancomycin and Linezolid. Klebsiella pneumoniae showed highest sensitivity to Amikacin 57(76). Among Klebsiella pneumoniae 31(41.33) were ESBL Producers . Among the  Escherichia coli isolates 24(55.81) were confirmed to be ESBL producers. Among the Proteus vulgaris isolates 5(38.46) were ESBL producers. Among the MRSA 46(62.16) were biofilm  producers. 12(38.7) ESBL strains of Klebsiella pnuemoniae , 11(45.83) ESBL strains of Escherichia coli and 5(100) ESBL strains of Proteus vulgaris were biofilm producers. Out of 357 isolates from wound infection samples 260(72.8) were biofilm producers. The predominant isolate producing biofilm was Staphylococcus aureus 83(62.40) followed by Klebsiella pnuemoniae 56(74.66). Detection of biofilm producers and their sensitivity pattern is of immense value in selecting the                 appropriate antibiotic for effectively treating wound infections.