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Phytochemical Analysis of Successive Extract of Curcumin and Comparing it with Standard Curcumin using High Performance Thin Layer Chromatography

PRIYADHARSINI .

Abstract


Aim Standardizing the curcumin extract for preclinical studies. ObjectivesPreparation of turmeric extract and           standardization using commercially available                           curcumin .Qualitative and quantitative estimation of curcumin content in the extract using HPTLC as Standardization method. Materials and Methods Turmeric obtained from Coimbatore and authentified at Botanical Survey India was pretreated and then extracted using hot percolation process with chloroform and ethanol in soxhlet apparatus. Concentrated ethanolic extract was then standardized by comparing it with commercially              available curcumin using High performance thin layer                 chromatography(HPTLC).Results Retention factor(Rf) value of our extract was found to be exactly similar to commercially  available curcumin. The quantity of curcumin in the extract was found to be 48.27mg per gm. Conclusion Thus to conclude  standardization of herbal products is essential before any             preclinical study and using HPTLC for standardization is simple and sensitive.

 


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