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A STUDY ON BACTERIAL PROFILE AND ANTIBIOTIC SUSCEPTIBILITY PATTERN IN POST SURGICAL WOUND INFECTION IN A TERTIARY CARE CENTRE

NATESAN BALAGURU

Abstract


OBJECTIVE To study the bacterial profile
and antibiotic susceptibility pattern in post
surgical wound infections.MATERIALS
AND METHODS This study was done in
patients with post surgical wound infections.
Pus samples were collected from
wound discharge using sterile swab and
processed by Gram staining and culturing
on to appropriate plates.Antibiotic susceptibility
testing was done by Disc diffusion
method (Kirby Bauer method) on Mueller
Hinton Agar . RESULTS Out of 50 samples,
54 organisms were isolated. E.coli
was the predominant isolate( 29.6 percentage),
followed by Pseudomonas aeruginosa
( 22.2 percentage) . E.coli was 31.3
percentage sensitive to Ceftazidime, 43.8
percentage sensitive to Ofloxacin, 93.8
percentage sensitive to Amikacin and were
100 p-ercentage sensitive to Cefoperazone
sulbactum and Imipenem . Pseudomonas
aeruginosa was 16.7 percentage
sensitive to Ceftazidime Ofloxacin,41.7
percentage sensitive to Amikacin,91.7 percentage
sensitive to
Cefoperazone sulbactum and 100 percentage
sensitive to Imipenem.Klebsiella
pneumoniae was 66.7 percentage sensitive
to Amikacin and Ofloxacin but only
16.7 percentage sensitive to Ceftazidime.
It was 100 percentage sensitive
to Cefoperazone sulbactam and
Imipenem.Staphylococcus aureus was
100 percentage sensitive to Amikacin and
62.5 percentage sensitive to Erythromycin
and Cephalexin and 12.5 percentage sensitive
to Ciprofloxacin.Staphylococcus epidermidis
was 50 percentage sensitive to
Amikacin ,Cotrimoxazole,Ciprofloxacin
and Tetracycline.Only one Enterococcus
faecalis was isolated and it was sensitive
to Amikacin, Ciprofloxacin and Penicillin.
CONCLUSION High resistance rates
has been observed for Cephalosporins
and Fluoroquinolones among the Gram
negative bacilli in post surgical wound infections.
Amikacin ,Cefoperazone sulbactum
and Imipenem were found effective in
these organisms and they will be effective
in formulating the prophylactic therapy for
prevention of post surgical wound infection.


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References


Archibald , J., and Blackely C.L. Canine

surgery 2nd edition. American Veterinary

Publication.Santa Barbara, California,

, p.17.

Chah .K.F., Eze .C.A, and Oluoha.B.N.

,Frequency and antimicrobial resistance

of aerobic bacteriaisolated from surgical

sites in humans and animals in NSUKKA,

Southeast Nigeria., NigerianVeterinary

Journal Vol. 24 [1] 1-7.

Chaudhary U, Aggarwal R [2004]. Extended

spectrum-lactamases [ESBL] – An

emerging threat toclinical therapeutics.

Indian J. Med Microbiol., 22: 75-80.

Culver DH, Horan TC, Gayenes RP et

al. Surgical wound infection rates by

wound class, operativeprocedure, and

patient risk index. National Nosocomial

Infections Surveillance System. Am J

Med1991; 91(3B):152S-157S.

Frank E.R. Veterinary Surgery; 4th edn.

Burgess Publishing company , Minneapol

i s , [ 1 9 6 0 ] p p . 1 3 -

.

Gales ,A.C., Ronald N.J., Michael .a

Pfaller A., Hwlie ,S., and Sentary study

Group[200]. Two- yearassessment of the

pathogen frequency and antimicrobial resistance

patterns among organismsisolated

from skin and soft tissue infections

in Latin American Hospitals. Int . J. Infectious

Diseases 4:75-76.

K.R.Sanjay, M.N.Nagendra Prasad and

G.S.Vijayakumar ., A study on isolation and detection

ofdrug resistance gram negative

bacilli with special importance to post

operative wound infection .,Journal of

Microbiology and Antimicrobials Vol-2

,pp. 68-75, Sep-2010 .

Leape LL. ,Brennan TA. Laird N et al.

The nature of adverse events in hospitalized

patients. Resultsof the Harvard

Medical practice study II. N. Engl J Med

;324[6]:377-384

Mangram AJ, Horan TC, Pearson MI,

Silver I.C, Javis WR, Guideline for prevention

of surgical siteinfection, 1999.

Hospital Infection Control Practices Advisory

Committee. Infect Control

HospEpidemiol 1999; 20:250-78.

Nathens AB, Cook CH. Machiedo G,

Moore EE, Namias N. Defining the research

agenda for surgical infection: a

consensus of experts using the Delphi

approach. Surg Infect [Larchmt] 2006; 7

(2):101-110 .

Nutanbala .N.Goswami, Hiren .R.

Trivedi,Antibiotic sensitivity profile of

bacterial pathogens in postoperative

wound infections at a tertiary care hospital

in Gujarat, India. ,Journal of Pharmacology

andPharmacotherapeutics ,July-

September 2011,Vol-2 –issue-3 .

Smith H.A., Jones, T.C. Hunt R.D.

Veterinary Pathology, 4th edn. Lea and

F e b r i g e r , P h i l a d e l p h i a ,

: p .581.

Suzanne M. Pear.R.N., Patient risk

factors and best practices for surgical

site infection prevention,Managing infection

control ,Education and training .

4 . T h o m a s R . T a l b o t , A l l e n

B.Kaiser.,Post operative infections and

antimicrobial prophylaxis, Mandell,

Douglas,Benett’s Principles and

Practice of infectious diseases , 6 th

ed, Elsevier Philadelphia 2005.Chapter

: page-3533-45.


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