University Journal of Pre and Paraclinical Sciences
CURRENT TRENDS OF ANTIBIOTIC RESISTANCE IN PSEUDOMONAS AERUGINOSA STRAINS IN TERTIARY CARE CENTER IN SOUTH INDIA
Abstract
PURPOSE OF THE STUDY To study the
Antibiotic susceptibility patterns of isolates
of Pseudomonas aeruginosa from hospitalized
patients. We also aim at finding out
the Extended Spectrum Beta Lactamase
(ESBL) and Metallo Beta Lactamase
(MBL) production in the isolates. MATERIALS
AND METHODS Out of 2552 bacterial
culture positive isolates in a period of 4
months (September-December,2011),
from various specimens of Inpatients ,197
(7.72 percent ) isolates of Pseudomonas
aeruginosa were isolated. These 197
Pseudomonas aeruginosa strains taken up
for the study. They were subjected to antibiotic
susceptibility testing by disc diffusion
test as per the Clinical and Laboratory
Standards Institute (CLSI) guidelines. Ceftazidime
resistant isolates were selected
for the detection of ESBL and MBL production
by phenotypic confirmatory test
and Modified Hodge test respectively. Appropriate
standard strains were used as
controls for antibiotic susceptibility testing,
ESBL and MBL detection.
Statistical analysis was carried out using
Statistical Package for Social Sciences
(SPSS) and Epi-info softwares by a statistician.
The proportional data of this study
were tested using Pearsons Chi square
analysis test 2.RESULTS Of the 197 isolates
of Pseudomonas aeruginosa studied,
31(15.7percent ) were ESBL producers
and 21(10.7 percent) were MBL producers.
Over all susceptibility was 91.4
percent for Imipenem, 80.2 percent for
Piperacillin-Tazobactam, 56.3 percent for
Ceftazidime, 45.7 percent to Amikacin,
44.7 percent to Ciprofloxacin, 42.1 percent
to Cefepime, 40.1 percent to Ofloxacin,
26.9 percent to Gentamicin and only
4.5 percent susceptibility to Piperacillin.
CONCLUSION Our findings showed
widespread emergence of drug resistance
among the Pseudomonas aeruginosa isolates.
Hence there is a need to do regular
surveillance to detect resistance patterns
and to use antibiotics judiciously and effectively,
so as to prevent their spread.
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