Determination of Bilirubin Interference In Creatinine Assay By Jaffe’s Method Among Chronic Kidney Disease Patients
Abstract
Abstract Creatinine measurement in icteric sample is a major problem due to negative interference of bilirubin over creatinine assay by Jaffe’s method. The objective of this study is to establish the negative interference caused by high bilirubin levels in creatinine estimation by Jaffe’s method. The data of 60 chronic kidney disease patientsamong which 30 patients had elevated bilirubin(group I) and 30 patients with normal bilirubin levels (group II) were included.The levels of Creatinine (Jaffe’s kinetic method),urea (kinetic method) and bilirubin(diazo method) in serum samples were measured in auto analyzer. Statistical analysis of quantitative data was done by using SPSS software version 24.0.
Results
Group I patients had a mean creatinine of 0.8mg/dL and bilirubin of 7.35mg/dL. Group II patients had a mean creatinine of 7.19mg/dl and bilirubin of 0.58 mg/dL. There was statistically significant negative interference due to bilirubin levels in creatinine assay among patients who had elevated bilirubin(group I). There was no correlation between the two parameters in the groupII patients where bilirubin were within normal limits. It was also observed that there is increased interference with increase in the concentration of the bilirubin levels.
Conclusion
In creatinine assay by Jaffe’s method, negative interference of high bilirubin has been established. From our study the cut off value of bilirubin interference by ROC has been estimated to be 7.85mg/dL.
Key Words:Bilirubin, Creatinine, Jaffe’s method.
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