An in vitro study to compare the anti-oxidant properties of cardiovascular and anti-diabetic drugs
Abstract
Introduction: Oxidative stress plays an important role in various pathological conditions like Diabetes mellitus, Hypertension, Malignancies, Alzheimers disease, etc. Scientific evidence suggests that antioxidants reduce the risk of these chronic diseases.2 There are numerous cardiovascular and anti-diabetic drugs available which have additional antioxidant properties. The aim of this study is to comparatively evaluate the antioxidant properties of cardiovascular drugs like Nebivolol, Carvedilol, Cilnidipine, Atorvastatin and that of anti-diabetic drugs like Sitagliptin, Vildagliptin and Tenegliptin by their free radical scavenging property using DPPH (2,2-diphenyl 1-picryl hydrazyl hydrate) assay. Methodology: Oral formulations of Nebivolol, Carvedilol, Cilnidipine, Atorvastatin, Sitagliptin, Vildagliptin, Teneligliptin were crushed separately into fine powders and stock solutions of 1000 and 500 μg/ml were prepared using ethanol. Ascorbic acid was used as the reference antioxidant. The percentage of antioxidant activity was assessed by DPPH assay method described by Brand Williams et al. Results: At concentrations of 1000 μg/ml and 500 μg/ml, the percentage of DPPH inhibition by the drugs were: Nebivolol - 46.38% and 35.28%, Carvedilol - 59.58% and 35.74%, Cilnidipine - 64.37% and 54.67%, Atorvastatin - 69.39% and 47.55%, Teneligliptin - 68.81% and 53.74%, Vildagliptin - 63.90% and 50.58% and Sitagliptin - 53.86% and 36.21% respectively. The percentage of DPPH inhibition for the same concentration of Ascorbic acid was 98.83% and 70.44% respectively. Conclusion: Our study has demonstrated the in vitro anti-oxidant activity of the commonly used cardiovascular drugs (Nebivolol, Carvedilol, Cilnidipine and Atorvastatin) and antidiabetic drugs (Teneligliptin, Vildagliptin and
Sitagliptin) which could have a therapeutic advantage of preventing oxidative stress induced complications in patients who are on long term treatment for cardiovascular diseases and diabetes mellitus.
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