Effect of Metformin and Hydroxy Chloroquine Combination on Blood Glucose in Streptozotocin Induced Diabetic Rats

Abstract

Aim and Objective : To investigate the effect of                         Hydroxychloroquine (HCQ) alone and in combination with Metformin on glycemic control on Streptozotocin  induced diabetic rats.

 Methodolody :  24 inbred adult male albino rats weighing 150 to 200 grams from central animal house, Madurai were utilized for the study. They will be divided into 4 groups of 6 animals each. Animals were allowed feed on standard diet (pellet feed) , tap water ad libitum. Streptozotocin was used to induce diabetes in the albino rats.  Streptozotocin was given at the dose of 60mg/kg intraperitoneally as a single dose after          overnight fasting. Group 1 (normal control) consisted of          normal rats that neither received any drug. Rats in Group 2 were diabetic and treated with Metformin 200mg/kg         orally. Rats in Group 3 were diabetic and treated with              Hydroxychloroquine  200 mg/kg orally . Animals in Group 4 were diabetic and treated with combination of Metformin and Hydroxychloroquine(200 mg/kg orally . The drugs were given once daily for 14 days.

Results : In Streptozotocin induced diabetic rats,                Hydroxychloroquine  with Metformin combination therapy reduced blood glucose level significantly (P< 0.001)  then used alone.

Conclusion : Outcomes of this investigation indicate that combination of Metformin with HCQ improves glycemic control and provides additional metabolic benefits, not achieved with either HCQ or metformin used alone. The  combination of Metformin and Hydroxychloroquine exhibited the most               significant reduction in glucose level by its peripheral action i.e, extrapancreatic action.

Key Words :  Metformin, Hydroxychloroquine, Streptozotocin, blood glucose, glucometer