Clinical Significance of Anti Annexin V IgG Antibodies in Systemic Lupus Erythematosus
Abstract
Background: Annexin V is an anionic phospholipid binding protein which has anticoagulant property by inhibiting prothrombin activity. Anti Annexin V antibodies have been found to be associated with thrombotic events and fetal miscarriage in patients of systemic lupus erythematosus (SLE).
Objective: The objective of the study is to determine the clinical significance of anti Annexin V IgG antibodies in patients with systemic lupus erythematosus.
Material and Methods: A cross sectional study was done including 100 consecutive SLE patients who satisfied 2012 SLICC criteria for SLE. 40 age and sex matched healthy controls were taken. Sera were analyzed for anti Annexin V IgG antibodies by ELISA.
Results: Of the total 100 patients, 9 patients (9%) showed positive for anti Annexin V IgG antibodies. The significant elevation of anti Annexin V IgG antibodies (p=0.0016) was seen in SLE patients with antiphospholipid antibody syndrome than in those SLE patients without antiphospholipid antibody syndrome. SLE patients who had unfavourable fetal outcome showed significant higher levels of anti Annexin V IgG antibodies when compared to those who had favourable fetal outcome (p=0.009). Significant higher levels of anti Annexin V IgG antibodies were detected in patients with one or more thrombotic events than those without thrombotic events (p=0.001).
Conclusion: This study emphasis the significant presence of anti Annexin V IgG antibodies in relation to APS, unfavourable fetal outcome and thrombotic events in SLE.
Keywords: Annexin V, thrombotic events, antiphospholipid antibody syndrome, unfavourable fetal outcomeFull Text:
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Khamasha MA, Amigo MC. Rheumatology. 10th ed: Elsevier publication; 2017. p1144.
Oosting JD, Derksen RH, Bobbink IW, et al. Antiphospholipid antibodies directed against a combination of phospholipids with prothrombin, protein C or protein S: an explanation for their pathogenic mechanism? Blood 1993; 18:2625.
Levine JS, Branch DW, Rauch J. The antiphospholipid syndrome. N Engl J Med 2002; 346: 752-63.
Gaëtan Espositoa B, Mathieu C, Tambya, et al. Anti-annexin V antibodies: are they prothrombotic? Autoimmunity Reviews 2005; 4:55–60.
Rand JH, Wu XX, Guller S, Scher J, Andree HAM, Lockwood CJ. Antiphospholipid immunoglobulin G antibodies reduce annexin-V levels on syncytiotrophoblast apical membranes and in culture media of placental villi. Am J Obstet Gynecol 1997; 177:918–23.
Wang X, Campos B, Kaetzel MA, Dedman JR. Annexin V is critical in the maintenance of murine placental integrity. Am J Obstet Gynecol 1999; 180: 1008–16.
Nojima J, Kuratsune H, Suehisa E. Association between the prevalence of antibodies to beta(2)-glycoprotein I, prothrombin, protein C, protein S, and annexin V in patients with systemic lupus erythematosus and thrombotic and thrombocytopenic complications. Clin Chem 2001; 47:1008– 15.
Rodriguez-Garcia MI, Fernandez JA, Rodriguez A, Fernandez MP, Gutierrez C, Torre-Alonso JC. Annexin V autoantibodies in rheumatoid arthritis. Ann Rheum Dis 1996; 55: 895–900.
Sugiura K, Muro Y. Anti-annexin Vantibodies and digital ischemia in patients with scleroderma. J Rheumatol 1999; 26: 2168-72.
Tripathy NK, Sinha N, Nityanand S. Antiannexin V antibodies in Takayasu’s arteritis: prevalence and relationship with disease activity. Clin Exp Immunol 2003; 134:360–4.
Matsuda J, Gotoh M, Saitoh N. Antiannexin antibody in the sera of patients with habitual fetal loss or preeclampsia. Thromb Res 1994; 75:105-106.
Miyakis S, Lockshin MD, Atsumi T et al. International consensus statement on an update of the classification criteria for definite antiphospholipid syndrome (APS). J Thromb Haemost 2006; 4: 295-306.
Van Heerde WL, de Groot PG, Reutelingsperger CPM.The complexity of the phospholipid binding protein annexin V. Thromb Haemost 1995; 73:172-9.
Gerke V, Creutz CE, Moss SE. Annexins: linking Ca2+ signalling to membrane dynamics. Nat Rev Mol Cell Biol 2005; 6: 449–61.
Van Genderen HO, Kenis H, Hofstra L, Narula J, Reutelingsperger CP. Extracellular annexin A5: functions of phosphatidylserine-binding and two dimensional crystallization. Biochem Biophys Acta 2008; 1783: 953–63.
Nakamura N, Shidara Y, Kawaguchi N, et al. Lupus anticoagulant autoantibody induces apoptosis in umbilical vein endothelial cells: involvement of annexin V. Biochem Biophys Res Commun 1994; 205:1488–93.
Satoh A, Suzuki K, Takayama E. Detection of anti-annexin IV and V antibodies in patients with antiphospholipid syndrome and systemic lupus erythematosus. J Rheumatol 1999; 26:1715– 20.
Kaburaki J, Kuwana M, Yamamoto M, Kawai S, Ikeda Y. Clinical significance of Anti-Annexin V Antibodies in Patients with Systemic Lupus Erythematosus. Am J Hematology 1997; 54: 209-213.
Matsuda J, Saitoh N, Gohchi K, Gotoh M, Tsukamoto M:Anti annexin V antibody in systemic lupus erythematosus patients with lupus anticoagulant and/or anticardiolipin antibody. Am J Hematology 1994; 47:56.
Bizzaro N, Tonutti E, Villalta D, Tampoia M, Tozzoli R. Prevalence and clinical correlation of anti-phospholipid-binding protein antibodies in anticardiolipin-negative patients with systemic lupus erythematosus and women with unexplained recurrent miscarriages. Arch Pathol Lab Med 2005; 129:61–8.
Ulcová-Gallová Z, Mukengnábl P, Hadravská S, Bibková K, Slechtová J, Kyselová V. Placenta and annexin V receptors, antibodies against annexin V and against other phospholipids in patients with recurrent pregnancy loss. Ceska Gynekol 2006; 71:469–73.
Nojima J, Kuratsune H, Suehisa E, et al. Association between the prevalence of antibodies to beta(2)-glycoprotein I, prothrombin, protein C, protein S, and annexin V in patients with systemic lupus erythematosus and thrombotic and thrombocytopenic complications. Clin Chem 2001; 47:1008–15.
Lakos G, Kiss E, Regeczy N, Tarjan P, Soltesz P, Zeher M, Bodolay E, Szucs G, Szakony S, Sipka S, Szegedi G. Antiprothrombin and Antiannexin V Antibodies Imply Risk of Thrombosis in Patients with Systemic Autoimmune Diseases. J Rheumatol 2000; 27: 924-9.
Zammiti W, Mtiraoui N, Kallel C, Mercier E, Almawi WY, Mahjoub T. A case-control study on the association of idiopathic recurrent pregnancy loss with autoantibodies against beta2-glycoprotein I and annexin V. Reproduction 2006; 131:817–22.
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